Carbenicillin
disodium salt
羧芐青霉素二鈉
C17H16N2Na2O6S MW 422.4 CAS: 4800-94-6
Synonym:
carbenicillin sodium; [2S-(2α,5α,6β)]-6-[carboxyphenylacetyl)amino]-3,3-
dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic
acid disodium salt;
α-carboxybenzylpenicillin
disodium
● Specification:
Appearance……………white
to off-white power
Solubility………………soluble
in H2O
Assay………………….>95%(dried basis)
● Product description:
Carbenicillin disodium is a
semi-synthetic β-lactam antibiotic that is related to penicillin. Its
bactericidal mode of action is analogous to that of benzylpenicillin, which
acts on bacteria by inhibiting bacterial cell wall synthesis. Relative to
benzylpenicillin, carbenicillin has a relatively greater spectrum of activity
against Gram-negative bacteria such as Pseudomonas
aeruginosa, but a lower activity against Gram-positive bacteria. Commonly
used for the elimination of Agrobacterium species after inoculation.
● 使用方法:
1. 儲液配制(水溶解):
|
1 g
|
5 g
|
500 mg/ml(1.18 M)
|
2 ml
|
10 ml
|
注:配好溶液后用0.22 μm抽濾除菌,-20℃貯存,有效期7天;4-8℃貯存,有效期3天;-80℃貯存有效期一個(gè)月。該產(chǎn)品在溶液狀態(tài)不穩定,建議您現用現配,即刻使用。
2. 植物共培養中使用濃度:
農桿菌
|
材料
|
羧芐濃度 μg/ml
|
頭孢噻肟鈉濃度μg/ml
|
Timentin濃度
μg/ml
|
文獻
|
土壤農桿菌
|
馬鈴薯葉盤(pán)
|
400-600
|
No
data
|
No
data
|
甘肅農業(yè)大學(xué)學(xué)報 2002,Vol
37,92-94
|
發(fā)根農桿菌LBA9402
|
茶樹(shù)組培芽
|
300-500
|
300-500
|
200-400
|
茶葉科學(xué),2015,35(3),437-442
|
發(fā)根農桿菌15834
|
茶樹(shù)組培芽
|
17天長(cháng)菌,不能有效抑制
|
300-500
|
200-400
|
茶葉科學(xué),2015,35(3),437-442
|
根瘤農桿菌LBA4404
|
藍莓
|
200-400
|
No
data
|
No
data
|
北方園藝,2010,16,135-137
|
根瘤農桿菌EHA105
|
油菜
|
500
|
No
data
|
No
data
|
西北植物學(xué)報,2005年第1期52-56
|
根瘤農桿菌LBA4404
|
油菜
|
200
|
No
data
|
No
data
|
西北植物學(xué)報,2005年第1期52-56
|
根瘤農桿菌LBA4404
|
大豆子葉
|
No
data
|
300
|
No
data
|
廣東農業(yè)科學(xué),2011,38,76-78
|
根瘤農桿菌AGL1
|
LB平板
|
此菌株含羧芐抗性,在500
μg/ml的平板上正常生長(cháng)
|
No
data
|
No
data
|
Real-Times
|
根瘤農桿菌EHA105
|
LB平板
|
500
|
No
data
|
No
data
|
Real-Times
|
根瘤農桿菌LBA4404
|
煙草
|
250
|
250
|
500
|
Plant Cell Reports
(1998) 17: 646–649
|