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首頁(yè) > 蛋白生物學(xué) > 蛋白Marker > 多肽電泳Marker

彩虹預染超低分子量蛋白Marker(1.2-45KD)

彩虹預染超低分子量蛋白Marker(1.2-45KD)

產(chǎn)品編號:RTD6110

產(chǎn)品規格:10次

數量
價(jià)格 ¥500

彩虹預染超低分子量蛋白質(zhì)Marker1.2-45kD

Rainbow Prestained Ultra Low Molecular Weight Protein Marker

 

產(chǎn)品編號及規格:

RTD6110   10T50μl)   500元

儲存條件:

  -20保存,開(kāi)封后有效期12個(gè)月

產(chǎn)品簡(jiǎn)介:

    彩虹預染超低分子量蛋白質(zhì)Marker可以直接觀(guān)察蛋白電泳及清晰判斷Western Blotting的轉膜效果。本產(chǎn)品包含3種多肽和3種低分子量蛋白質(zhì)組成,分子量范圍為1.2kD-45kD,分子量大小為  1.2kD,4.6kD,10kD,16kD,27kD,45kD (注:蛋白大小已在18%Tricine-SDS-PAGE中用非預染蛋白Marker標定過(guò)。


● 使用說(shuō)明:

第一次收到該產(chǎn)品,常溫融化后,徹底混勻,離心快甩將溶液完全收集到管底。

. 制膠:

I 配制分離膠

1.按照表一將不同體積的雙蒸水、40% PAA19:1)、4×凝膠緩沖液(貨號:GB010)和乙二醇加入到小燒杯中混合。

2.加入10%APSTEMED,立即混勻5-10秒,以使溶液充分混勻。

3.在凝膠模具中迅速灌入適量分離膠溶液,然后在分離膠溶液上輕輕覆蓋一層1 cm的無(wú)水乙醇,使凝膠表面保持平整。

4.靜置15-30分鐘,待分離膠和乙醇層之間出現一個(gè)清晰的界面表示凝膠已聚合。

表一  (一塊1 mm mini膠用量)

 

分離膠

濃縮膠

 

18T, 5%C /5 ml

5T, 3.3%C/2 ml

40% PAA19:1

2.25 ml

/

40% PAA (29:1)

/

0.25 ml

4×凝膠緩沖液

1.25 ml

0.5 ml

乙二醇(電泳級)

1.5 ml

/

ddH2O

/

1.25ml

10APS

50 μl

20 μl

TEMED

5 μl

2 μl


II 配制濃縮膠

去除覆蓋在分離膠上的乙醇層,用濾紙將殘留乙醇吸去。

1.按照表一將不同體積的雙蒸水、40%PAA(29:1)和4×凝膠緩沖液加入到小燒杯中混合。

2.加入10%過(guò)硫酸銨和TEMED,立即混勻5-10秒,以使溶液充分混勻。

3.將梳子插入凝膠內,避免產(chǎn)生氣泡。

4.靜置30-60分鐘裝待凝膠聚合

. 電泳:

1. Marker樣品,充分混勻后備用。不要加熱處理。

2. 電泳前,將10×陽(yáng)極緩沖液(Cat No:AB080)和10×陰極緩沖液(Cat No:CB010)用蒸餾水稀釋成1×緩沖液備用。將電泳槽的外槽加入1×陽(yáng)極緩沖液,內槽加入1×陰極緩沖液,輕柔拔出梳子,將Marker或蛋白樣品(已經(jīng)過(guò)2×Tricine上樣緩沖液[Cat No:TP050]處理)加入點(diǎn)樣孔,參考下表進(jìn)行電泳,至每條帶都清晰可見(jiàn)時(shí)停止電泳。整個(gè)電泳過(guò)程大約需要2-3個(gè)小時(shí)。

表二 多肽電泳條件

恒電壓

150V

起始電流

60-75mA/板膠

結束電流

15-25mA/板膠

電泳時(shí)間

2.5-3小時(shí)

. 染色

根據常規實(shí)驗步驟,用配方6和7(表三)進(jìn)行染色和觀(guān)察。如果使用配方6進(jìn)行染色時(shí)效果不好或考慮其毒性,請選擇本公司的FastBlue蛋白染色液(貨號:RTD6202),該產(chǎn)品除具有染色快,無(wú)毒,靈敏性高等特點(diǎn)外,還能對小肽在染色中起到固定作用,不至于小肽在染色和脫色中從凝膠中脫離,是常規染色液的理想替代品。


相關(guān)產(chǎn)品:


多肽電泳配套試劑

名稱(chēng)

貨號

規格

超低分子量蛋白電泳試劑盒

RTD6120

10

超低分子量蛋白Marker I3.3-20.1 kD

RTD6101

10次(100 μl

超低分子量蛋白Marker III3.4-100 kD

RTD6125

10次(50 μl

彩虹預染超低分子量蛋白Marker1.2-45 kD

RTD6110

10 50 μl

彩虹預染超低分子量蛋白Marker II3-40 kD

RTD6145-01

10 50 μl

40% PAA19:1

AC1914-01

100ml

40% PAA29:1

AC2914-01

100ml

多肽電泳凝膠緩沖液

GB010-100ml

100ml

2×Tricine 上樣緩沖液(變性,還原)

TP050

10×1ml

FastBlue蛋白染色液

RTD6202

500ml

乙二醇(電泳級)

EA0582-02

100 ml

10×Tris-Tricine-SDS緩沖液(變性電泳,粉末型)

CB010P

500 ml

10×陽(yáng)極緩沖液 (多肽電泳用

AB080

250 ml

10×陰極緩沖液(多肽電泳用)

CB010

100 ml

 


使用RTD6110 彩虹預染超低分子量蛋白Marker1.2-45KD產(chǎn)品發(fā)表部分文章列表:

1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieae isolated from traditional Chinese fermented cucumber.

Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Food Control 50 (2015) 896e900

Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper linkhttp://dx.doi.org/10.1016/j.foodcont.2014.10.040

 

2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by   isolated from fermented sausage Lactobacillus plantarum.

Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie

Zhang, Changqing Yu, Xue Han

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Food Control  Volume 81, November 2017, Pages 211-217

Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper linkhttps://doi.org/10.1016/j.foodcont.2017.05.030

 

3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Frontiers in Plant Science.  Volume 81, November 2017, Pages 211-217

InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper linkhttps://doi.org/10.1093/jxb/ery351

 

4. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu

Marker: RTD6110,彩虹超低分子量蛋白Marker (1.2-45 kD)

Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019,613-626

InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper linkhttps://doi.org/10.1093/jxb/ery351

 

5. [2021 IF=11.277] An  Alternative  Splicing  Variant  of  PtRD26  Delays  Leaf  Senescence by Regulating Multiple NAC Transcription Factors in Populus.

Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li

Marker: RTD6110,彩虹預染超低分子量蛋白(1.2-45 kD)

Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614

InstitutionBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University

Paper linkhttps://doi.org/10.1093/plcell/koab046

 

6. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Author: Feng Qin, Shujuan Wang, Mingxia Gao  and  Xiangmin Zhang

Marker: RTD6110,彩虹預染超低分子量蛋白(1.2-45 kD)

Journal: Analytical Methods. 2021,13, 2804-2811

InstitutionDepartment of Chemistry, Fudan University

Paper linkhttps://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b

 

7. [2022 IF=6] Peptide LQLY3-1, a novel Vibrio harveyi quorum sensing inhibitor produced by Lactococcus lactis LY3-1.

Author: Yangrui Wang, Mengtong Sun, Xiaoling Cui, Yongyue Gao, Xinran Lv, Jianrong Li, Fengling Bai, Xuepeng Li, Defu Zhang, Kai Zhou

Marker: RTD6110,彩虹預染超低分子量蛋白(1.2-45 kD)

Journal: LWT - Food Science and Technology 170 (2022) 114093

InstitutionCollege of Food Science and Engineering, Bohai University

Paper linkhttps://doi.org/10.1016/j.lwt.2022.114093

 


 
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国产三级精品三级在线观看,久久久久久久久,久久偷看各类wc女厕嘘嘘偷窃,中文字幕日韩一区二区三区不卡