彩虹預染超低分子量蛋白質(zhì)Marker(1.2-45kD)
Rainbow Prestained Ultra Low Molecular Weight
Protein Marker
●產(chǎn)品編號及規格:
RTD6110 10T(50μl) 500元
● 儲存條件:
-20℃保存,開(kāi)封后有效期12個(gè)月
● 產(chǎn)品簡(jiǎn)介:
彩虹預染超低分子量蛋白質(zhì)Marker可以直接觀(guān)察蛋白電泳及清晰判斷Western Blotting的轉膜效果。本產(chǎn)品包含3種多肽和3種低分子量蛋白質(zhì)組成,分子量范圍為1.2kD-45kD,分子量大小為 1.2kD,4.6kD,10kD,16kD,27kD,45kD (注:蛋白大小已在18%Tricine-SDS-PAGE中用非預染蛋白Marker標定過(guò)。
● 使用說(shuō)明:
第一次收到該產(chǎn)品,常溫融化后,徹底混勻,離心快甩將溶液完全收集到管底。
一. 制膠:
I 配制分離膠
1.按照表一將不同體積的雙蒸水、40% PAA(19:1)、4×凝膠緩沖液(貨號:GB010)和乙二醇加入到小燒杯中混合。
2.加入10%APS和TEMED,立即混勻5-10秒,以使溶液充分混勻。
3.在凝膠模具中迅速灌入適量分離膠溶液,然后在分離膠溶液上輕輕覆蓋一層1
cm的無(wú)水乙醇,使凝膠表面保持平整。
4.靜置15-30分鐘,待分離膠和乙醇層之間出現一個(gè)清晰的界面表示凝膠已聚合。
表一 (一塊1 mm mini膠用量)
|
分離膠
|
濃縮膠
|
|
18%T, 5%C /5 ml
|
5%T, 3.3%C/2 ml
|
40% PAA(19:1)
|
2.25 ml
|
/
|
40% PAA (29:1)
|
/
|
0.25 ml
|
4×凝膠緩沖液
|
1.25 ml
|
0.5 ml
|
乙二醇(電泳級)
|
1.5 ml
|
/
|
ddH2O
|
/
|
1.25ml
|
10%APS
|
50 μl
|
20 μl
|
TEMED
|
5 μl
|
2 μl
|
II 配制濃縮膠
去除覆蓋在分離膠上的乙醇層,用濾紙將殘留乙醇吸去。
1.按照表一將不同體積的雙蒸水、40%PAA(29:1)和4×凝膠緩沖液加入到小燒杯中混合。
2.加入10%過(guò)硫酸銨和TEMED,立即混勻5-10秒,以使溶液充分混勻。
3.將梳子插入凝膠內,避免產(chǎn)生氣泡。
4.靜置30-60分鐘裝待凝膠聚合
二. 電泳:
1. 取Marker樣品,充分混勻后備用。不要加熱處理。
2. 電泳前,將10×陽(yáng)極緩沖液(Cat No:AB080)和10×陰極緩沖液(Cat No:CB010)用蒸餾水稀釋成1×緩沖液備用。將電泳槽的外槽加入1×陽(yáng)極緩沖液,內槽加入1×陰極緩沖液,輕柔拔出梳子,將Marker或蛋白樣品(已經(jīng)過(guò)2×Tricine上樣緩沖液[Cat No:TP050]處理)加入點(diǎn)樣孔,參考下表進(jìn)行電泳,至每條帶都清晰可見(jiàn)時(shí)停止電泳。整個(gè)電泳過(guò)程大約需要2-3個(gè)小時(shí)。
表二 多肽電泳條件
恒電壓
|
150V
|
起始電流
|
60-75mA/板膠
|
結束電流
|
15-25mA/板膠
|
電泳時(shí)間
|
2.5-3小時(shí)
|
三. 染色
根據常規實(shí)驗步驟,用配方6和7(表三)進(jìn)行染色和觀(guān)察。如果使用配方6進(jìn)行染色時(shí)效果不好或考慮其毒性,請選擇本公司的
FastBlue蛋白染色液(貨號:RTD6202),該產(chǎn)品除具有染色快,無(wú)毒,靈敏性高等特點(diǎn)外,還能對小肽在染色中起到固定作用,不至于小肽在染色和脫色中從凝膠中脫離,是常規染色液的理想替代品。
相關(guān)產(chǎn)品:
多肽電泳配套試劑
使用RTD6110 彩虹預染超低分子量蛋白Marker(1.2-45KD)產(chǎn)品發(fā)表部分文章列表:
1. [2021 IF=5.5] Garviecin LG34, a novel
bacteriocin produced by Lactococcus
garvieae isolated from traditional Chinese fermented cucumber.
Author: Yurong Gao, Dapeng Li, Shan Liu,
Liyuan Zhang
Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)
Journal: Food Control 50 (2015) 896e900
Institution:Key Laboratory of Veterinary Medicine
of Heilongjiang Bayi Agricultural University
Paper link:http://dx.doi.org/10.1016/j.foodcont.2014.10.040
2. [2021 IF=5.5] Purification and partial
characterization of M1-UVs300, a novel bacteriocin produced by isolated from fermented sausage Lactobacillus
plantarum.
Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi,
Zhaohang Zuo, Xiaoxi Xu, Dongjie
Zhang,
Changqing Yu, Xue Han
Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)
Journal: Food Control Volume 81, November 2017, Pages
211-217
Institution:Key Laboratory of Veterinary Medicine
of Heilongjiang Bayi Agricultural University
Paper link:https://doi.org/10.1016/j.foodcont.2017.05.030
3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like
Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.
Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie
Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu
Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)
Journal: Frontiers in Plant Science. Volume 81, November 2017, Pages
211-217
Institution:Institute of Plant
Protection, Chinese Academy of Agricultural Sciences
Paper link:https://doi.org/10.1093/jxb/ery351
4. [2021 IF=6.99] Verticillium dahliae Alt a
1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal
infection.
Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie
Dong, Xiufen Yang, Dewen Qiu
Marker: RTD6110,彩虹超低分子量蛋白Marker
(1.2-45 kD)
Journal: Journal of Experimental Botany, Vol
70, Issue 2, 15 January 2019,613-626
Institution:Institute of Plant
Protection, Chinese Academy of Agricultural Sciences
Paper link:https://doi.org/10.1093/jxb/ery351
5. [2021 IF=11.277] An Alternative
Splicing Variant of
PtRD26 Delays Leaf
Senescence by Regulating Multiple NAC Transcription Factors in Populus.
Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi
Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia,
Hongwei Guo, and Zhonghai Li
Marker: RTD6110,彩虹預染超低分子量蛋白(1.2-45 kD)
Journal: Plant Cell. Volume 33, Issue 5, May
2021, Pages 1594–1614
Institution:Beijing Advanced Innovation
Center for Tree Breeding by Molecular Design, Beijing Forestry University
Paper link:https://doi.org/10.1093/plcell/koab046
6. [2021 IF=2.89] Rapid and sensitive
detection ofStaphylococcus aureus and Klebsiella pneumonia based
on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted
laser desorption ionization-time of flight mass spectrometry.
Author: Feng Qin, Shujuan Wang, Mingxia Gao and
Xiangmin Zhang
Marker: RTD6110,彩虹預染超低分子量蛋白(1.2-45 kD)
Journal: Analytical Methods. 2021,13,
2804-2811
Institution:Department of Chemistry,
Fudan University
Paper link:https://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b
7. [2022 IF=6] Peptide LQLY3-1, a novel
Vibrio harveyi quorum sensing inhibitor produced by Lactococcus lactis LY3-1.
Author: Yangrui Wang, Mengtong Sun, Xiaoling Cui,
Yongyue Gao, Xinran Lv, Jianrong Li, Fengling Bai, Xuepeng Li, Defu Zhang, Kai
Zhou
Marker: RTD6110,彩虹預染超低分子量蛋白(1.2-45 kD)
Journal: LWT - Food Science and Technology 170
(2022) 114093
Institution:College of Food Science and
Engineering, Bohai University
Paper link:https://doi.org/10.1016/j.lwt.2022.114093